Transgenic Peanuts Resistant to Herbicides by Introducing the EPSPS Gene using Agrobacterium tumefaciens

Abstract

            A species of groundnut or peanut belongs to the fabaceae or Leguminosae family. Because of the pods that form in the soil, it gets its botanical name, Arachis hypogea, from the Greek words arachis, which means "legume," and hypogea, which means "below ground. Our research aims to create transgenic peanuts that are resistant to herbicides by introducing the EPSPS gene using Agrobacterium tumefaciens. figuring out how often explants that are co-cultivated on the appropriate shoot regeneration media for varying co-cultivation times transform. to look into the effects of phosphonothricine acetyl transferase (PAT) on explants and the ideal concentration for it.Shoot inducing, co-cultivation, selection and rooting media were used, binary plasmid pXCSG-mYFP, the disarmed Agrobacterium tumefaciens strain GV3101 served as the transformation vector system. Plant selectable genes were the phosphorus acetyl transferase (PAT) gene, which was driven by the CaMV 35S promoter and NOS terminator sequences, and YFP, which functioned as a reporter gene. The EPSPS gene of interest and the kanamycin resistance gene for bacterial selection were present in the plasmid pXCSG. The result showed that creating transgenic plants by Agrobacterium-mediated transformation is a suitable method. One technique for creating genetic variations is called "agrobacterium-mediated transformation." This approach could be extended to other species to boost the genetic diversity of the crops.